Water Soluble CdSe/ZnS Quantum Dots Synthesized as Fluorescent labels for Protein Detection using Lateral Flow Immunoassay (LFIA)
Aidil Fajri and Hye jin Lee*
CdSe cores was synthesized by non-injection and easily method for ZnS shell growth around CdSe cores in an organic solvent. The yellow emission color of quantum dots particles were transferred to water solutions by ligand exchange with mercaptopropionic acid for biological applications. A Lateral flow immunoassay (LFIA) technique was developed for the detection of proteins. A sandwich assay format involving two bioreceptors specific for the target molecules was designed. The protein detection was identified by the yellow line on the test line and control line under UV-lamp as the presence the target protein to form sandwich immunocomplexes on the lateral flow immunoassay strip.
Keywords: CdSe/ZnS,Quantum Dots, Fluorescent nanoparticle, LFIA, Detection protein,