It is important to develop a chip-based platform having excellent non-biofouling performances and high binding capability to target analytes. Thus, chemical functionalization on solid surfaces is required for fabricating chip-based biosensors. Polymeric coating with functionalizable, non-biofouling polymers is a good strategy to fabricate the surfaces. Among the polymers, polysaccharides are well-known as a non-biofouling material and have precursors for aldehyde functionality. In this study, agarose and amylose are employed to fabricate a protein chip where biotin-streptavidin interaction is chosen as a model study due to its high binding constant value. Each chip performance is evaluated using a fluorescence analysis, and several equipment are also employed to characterize the chips.