Rapid detection tests (RDTs) that can diagnose and monitor disease are important in health care. One of the RDTs methods is polymerization-based amplification (PBA). In PBA, it is well known that using xanthene dye in combination with a tertiary amine is effective. It is fast in detection and less susceptible to oxygen inhibitor. However, the detection limit is ppm level. In this study, to find out how to lower the detection limit, we measured the photophysical properties of xanthene dyes and compared their photopolymerization on the glass surface.