Fluorescent nucleoside analogs that are sensitive to their local environment, demonstrating changes in the fluorescent properties when induced by changes in polarity, pH, or even structure, have become powerful tools for the investigation of nucleic acid structure, dynamics, and recognition. Therefore we previously synthesized the fluorene-linked 2’-deoxyuridine through the Suzuki coupling of 5-iodo-2’-deoxyuridine with fluorene 2-boronic aicd pinacol ester. In this study, we synthesized fluorene-labeled 2’-deoxyuridine via a diacetylenyl linker and investigated its solvent-dependent photophysical properties. In addition, we will incorporate it into the central position of oligodeoxynucleotide to investigate the potential as a SNP probe upon the duplex formation with fully matched and single-base mismatched targets. Detailed research results including these will be presented.